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Julian Stirling
@julianstirling_gitlab
Hi @Matt319, the normal process is to mount the sample to the slide and then invert the slide. Clear nail polish and coverslips can be very helpful here. If the sample is too 3D then this may not be the best type of microscope
What is the sample?
Matt Asgari
@Matt319
@julianstirling_gitlab I was just looking at hair for the first test but the lens had to be pretty much touching the sample with the slide upside down
I can try the methods you suggested but is there no way to extend the gap between the objective and sample and retain focus (but loose some magnification I would assume)?
Julian Stirling
@julianstirling_gitlab
Are you using an RMS objective or the picamera lens?
Matt Asgari
@Matt319
I am using an RMS objective lens with pi camera 2
Julian Stirling
@julianstirling_gitlab
I think you are then limited to the parfocal distance of the objective. What magnification are you using?
It also may be affected by the focal length of the tube lens if it is not 50
Matt Asgari
@Matt319
60x
is there no way to adjust the distance between the objective and achromatic lens to change magnification but also focal length to the slide?
Julian Stirling
@julianstirling_gitlab
Oh yeah with a 60x you will be super close to the sample. A 60 also probably doesn't have the depth of field for a hair
Matt Asgari
@Matt319
When i looked at it i could adjust z to see different layers
it was pretty cool
is there a common objective lens that people use?
Julian Stirling
@julianstirling_gitlab
It really depends what you are looking at. For research we often use x100 for looking at red blood cells. It really depends how big and flat the thing you want to look at is
One downside of the OFM is the lack of space for a carousel of different objectives.
The microscope wins at great fine translation performance with high magnification. You can get lots of pretty cool looking pre-prepared slides if you want to do some fun microscopy with your 60x
Matt Asgari
@Matt319
Thanks for the info
i was also wondering when the led matrix update will be out?
Julian Stirling
@julianstirling_gitlab
I hope to merge the design files for the LED array into the master branch of the repository today. However, we will not be putting that in a patch release as there are significant changes to the underlying codebase underway and I am worried about stability. We will hopefully have an alpha release soon for testing. I need to talk to Sam about the status of the electronics and software for the array
ingramr88
@ingramr88
Does anyone mind sharing the GCODE for slicing the microscope body on a creality ender 3 pro? I use a broswer based slicer, IceSL, for the other parts, but when it comes to slicing the microscope body, its too big for IceSL to slice in my browser. My desktop computer is a raspbery pi. Any suggestions?
Daniel Rosen
@dgrosen2009_gitlab
Led array? Can you elaborate please?
Matt Asgari
@Matt319
Thanks @julianstirling_gitlab for the info
@dgrosen2009_gitlab it is an alternate form of lighting the sample. Instead of a single led it is a small matrix of LEDs
Get M
@GetM_gitlab
I was looking at sample but it looks 3D and not clear. what is the reason?
rob-arch
@rob-arch
Hi all, we have obtained images using the Delta stage model and seem to have a reflection of the camera appearing in focused images. Has anyone else experienced this problem or know how to fix this? Thanks
Julian Stirling
@julianstirling_gitlab
Did you print the optics module in black? If not it can both let light through and the light traps don't work well. This could perhaps illuminate the camera enough for this effect. Are you sure it is not an image of the LED? Do you have an example you can share? The forum may be best for this
rob-arch
@rob-arch
We printed the optics module and all other parts in black. Here is an example image, hopefully this helps. https://twitter.com/rob98arch/status/1347202572021600256
1 reply
rob-arch
@rob-arch
@julianstirling_gitlab Just wondering if you have any more suggestions with the imaging problem mentioned above
fv_flex flex
@fv_flex_gitlab
Hi all, question: I had an initial image which was transparant, then I ran several autocalibrates on the camera; but I keep getting a green image. Any ideas how to fix that issue?
1 reply
Daniel Rosen
@dgrosen2009_gitlab
That is intersting. I just downloaded the latest software tomfinish my 3rd OFM and the same thing happened. Green screen and sometimes weird lines. Cannot make it to callibrate right. Switched the camera and getting at network error.