@PTRRupprecht I'm still a little confused on the details, so to make sure I understand it correctly I'll describe the steps and indicate where I'm unsure. If you could correct me that would be very much appreciated.
Given an ROI from a series of TIFFs (e.g. one of the neurofinder datasets), I would take the following steps to get a spikefinder format column vector:
F_t = (F_0 - F_t) / F_0-- when I look at the datasets without preprocessing (https://goo.gl/GoEPfH), F_0 is much larger than the remaining values, but this isn't the case with the Neurofinder data, which makes me think I'm still misunderstanding this step.
preprocessfunction from c2s with fps/Hz = 100.
Is there maybe a snippet of code somewhere for converting a Neurofinder ROI into Spikefinder format?