@isaacovercast Great! The step 6 is working now. Thank you! By the way, I wonder whether a copy of the example data of tutorial Sub-sampling data sets could be deposited on the website of ipyrad of GitHub. Because currently place that the Dropbox is blocked in China, lots of people in China studying in RAD-seq is not easy to access the data on Dropbox. Of course, people can use VPN, but download will be terrible slow. If this will be very complex, just forget it. The ipyrad is already excellent!