These are chat archives for dereneaton/ipyrad

Oct 2017
Oct 31 2017 21:50
Dear @isaacovercast , @dereneaton . I have a question regarding reads with different length. I have PE ddrad data sequenced on 2 seperate instances and now all samples have PE reads of 75 and 150 bp length. I know iPyrad can handle reads with different length, but can it handle such big differences too? I prefer to do a denovo assembly. Would you expect to see 'funny' behaviour? I can imagine that the second 75 bps of loci have only half the coverage of the first 75 bases and might therefor cause problems. Do zou have any experience with this? Thank you for your help. Remco