@dereneaton @isaacovercast I have run my big data set separated in 44 files from step 1 to step 4 and now merged them to a new assembly. The all cluster files are about 36GB, I have run the merged assembly in step 5 on a HPC with about 800GB space left and the run stopped with error “no space left on device”. I want to know how many spaces needed at least when running the step5 to step7 or how many times spaces it needs compared to the space of cluster files? Have you ever simulated it? I need your help!