@isaacovercast Thanks! I tried vdb-config -p that seems print out configuration information about softwares under my account . I also tried to call some commands in the sratools without its full path and got right response, which means the sratolls folder has been in my default path file. Then, I check the sratools installed under anaconda again and ensured that sratools has been there in the conda list. Is it possible because of the file system of the hpc cluster I used? Like my last problem about hdf5 error. The sratools worked right in my local computer with bio-linux system but it will cost a lot of time because my computer isn't as powerful as the public hpc cluster in my institute.