@epnev , Thanks for sharing this software. It's been very useful for my work, especially in automating different aspects of analysis. One problem I've been hitting my head against is this: Some traces diverge quite wildly, giving me dF/F traces with values in the range of 10e5 and 10e6, in about 10-15% of the cases . This is in neurons that I can follow over several days, being mostly stable, then every once in a while a trace goes completely off the chart. Do you have any tips or tricks to tame these beasts? Where to look for problems? I'm aquiring at ~7Hz over 10s of minutes, and the signals in the converged recordings look very good.