These are chat archives for fiji/fiji

21st
Sep 2018
Varun Kapoor
@kapoorlab
Sep 21 2018 01:26
@tinevez @awalter17 I can probably use this code n modify it a bit to draw kth order polynomials in n-dimensional space, it wouldn't be exactly Bresenham algorithm though.
Varun Kapoor
@kapoorlab
Sep 21 2018 05:21
One extension would be to give k points and then fit line using @tinevez code to fit lines between an ordered set to get a piecewise linear polynomial, that would be Bresenham
Another extension could be to specify only two points and k - 2 parameters of the polynomial and then draw the polynomial between two points, that would require adding a penalty function based on distance of a point to a polynomial in the code of @tinevez to accept or reject the increment, while iterating over all points exactly once as in above.
Alison Walter
@awalter17
Sep 21 2018 07:27
@tinevez I think it is ok that it is not a "true" bresenham implementation, as long as we don't claim that it is. There are numerous ways to raster lines, so if at some point we discover cases that require a "true" bresenham or another line raster algorithm we can always implement such algorithms then. But that is just my opinion. Thoughts anyone else?
Alison Walter
@awalter17
Sep 21 2018 07:34
@kapoorlab cool! But we should probably see what we want to do with @tinevez 's implementation before we start extending it
massimiliano mellone
@mellmassi_gitlab
Sep 21 2018 09:27
hello, I am new to this chat. I need to ask the Fiji community something about Find edges. Can anybody help?
massimiliano mellone
@mellmassi_gitlab
Sep 21 2018 09:35
I am using this plugin to quantify fluorescently labelled actin fibres in my cells. I know this plugin is used for segmentation. I am just wondering if I can use it to quantify the intensity of the fibres measured as Gray values
Jan Eglinger
@imagejan
Sep 21 2018 10:46
@mellmassi_gitlab you'd usually first segment and then measure intensities on the original image, I guess. But that question needs more context, and ideally a sample image. Can you please ask it on the http://image.sc forum?
Curtis Rueden
@ctrueden
Sep 21 2018 15:01
@mellmassi_gitlab As a starting point, I suggest you check out https://imagej.net/Segmentation. That page describes typical ImageJ segmentation workflows. And then I second the suggestion to post on the image.sc forum.