"we created a simulated ground truth benchmark. We first ran the pipeline on a single experiment and manually curated the results.We then chose a small subset of cells (20 out of 300) and added their activity to a neighboring spatial location of the movie, which did not overlap significantly with the rest of the ROIs (Fig.
5a-b). We then re-ran the pipeline and compared the results with the 20 known cell locations.
We re-ran this test 43 times, choosing a different set of cells every time. The analysis shows
that almost all ground truth ROIs were correctly identified (Fig. 5c)"