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MelonaT
@MelonaT
Hi, I'm trying to use a workflow I created but I always get the bug report that "The server could not complete the request. Please contact the Galaxy Team if this error persists." What can I do?
Jennifer Hillman-Jackson
@jennaj
@MelonaT Hi -- please try at least one rerun. If the same error is reported, there is likely some problem with the inputs, tool choices, or workflow design. Try opening the workflow in the editor and check for warning messages. If you cannot resolve the problem, where are you running the workflow? URL if a public server or describe otherwise. Thanks!
@taikongrenren The tool is not finding a required dependency. This can be due to several reasons. Did you install Galaxy following the instructions here, with the latest release? https://galaxyproject.org/admin/get-galaxy/ Or by some other method?
MelonaT
@MelonaT
@jennaj thanks for your answer! I already did several reruns on different days, but I always got the same message. I can't find any warnings in the editor either. The point is, that when using every tool separately, everything works.... Here is the link to my workflow: https://usegalaxy.org/u/malena_trost/w/ba
I give an NCBI SRR number as input to the "Download and Extract Reads in FASTA/Q", than execute MEGAHIT and finally ABRicate.
11 replies
taikongrenren
@taikongrenren
@jennaj yes I followed the official tutorial to install that. I don't know the reason
4 replies
taikongrenren
@taikongrenren
I tried another way to use the command like below, still meet the same problem about "join"
7 replies
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Melisa Magallanes
@Melisa-Magallanes
Hi there! anybody else is getting a HTTP ERROR 500 at usegalaxy.org?
5 replies
Brian Thompson
@eye_science_bri_twitter
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Does anyone know why deseq produces a matrix with 10 genes when reading in data from sailfish?
13 replies
taikongrenren
@taikongrenren
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taikongrenren
@taikongrenren
Thanks@jennaj for your patient explanation. The command does works in the public server. I follow your suggestions to install another "join tool" and worked. Beside this when I used the tool goseq, my local server and the public server met a same question like
obove
github-ic
@github-ic
Hi - I'm using v21.01 and it's slow in uncompressing fasta.gz or fna.gz files via FTP. Works when uploading a few files but fails for 20 or more. The same uncompressed files load instantly. Has anyone else experienced this?
Melisa Magallanes
@Melisa-Magallanes
Hi!, can RNAMMER be installed?
selten
@selten
@Melisa-Magallanes On which URL are you working? Or which Galaxy?
Robert Leach
@hepcat72
Could anyone help me troubleshoot a failure of the sortmerna data manager?
I explain it here: bgruening/galaxytools#1146
Melisa Magallanes
@Melisa-Magallanes
@selten at usegalaxy.org
Robert Leach
@hepcat72
Hey @bgruening. Sorry to bother you, but do you have any insights into bgruening/galaxytools#1146 It seems I don't know enough about data managers to troubleshoot it.
muscle_body
@tepodone_twitter

@martenson @bgruening:matrix.org
Dear, Martin. Thank you for your advises. I have uploaded files what I use in X!tandem on Galaxy. Can you check them on the following GitHub URL?
https://github.com/m-and-o138/m-and-o138/compare/main...m-and-o138-patch-1?expand=1#diff-c0256b69eda82032850603f4263167eb8bdeee0ed8eb7a35a17b65c59115e95b

Additionally, I also indicated the error messages what I received when I executed the X!tandem on Galaxy.
m-and-o138/m-and-o138#1

I'm not still familiar with GitHub. So, I'm worry about whether you can check my files and error codes. If you could not check them, please contact me again. Thank you.

@martenson
@bgruening:matrix.org
Don't mean to be bothering you, but could you check my trouble mentioned above ?

bgruening
@bgruening:matrix.org
[m]
@tepodone_twitter I'm out this week, can you ping me.next week please? Sorry.
muscle_body
@tepodone_twitter
@bgruening:matrix.org Thank you for your reply. OK, I'll contact you later again.
godwinjames
@godwinjames
Get Data → UCSC Main , In the table browser I can't able download the gene annotation for Arabidopsis thaliana (TAIR 10). Is there any alternate ways to do it?
4 replies
FiReTiTi
@FiReTiTi
Hi, I'm a new user of Galaxy and I just wanted to make a test to create a tool, so I followed this tutorial: https://galaxyproject.org/admin/tools/add-tool-tutorial/
When I modified the file tool_conf.xml.mainto make Galaxy aware of the tool, it was not found, however, when I modified tool_conf.xml.sample it worked. Why?
Nuwan Goonasekera
@nuwang
@FiReTiTi Which file does the tool_config_file setting in your galaxy.yml file point to? By default, it will point to tool_conf.xml.sample.
FiReTiTi
@FiReTiTi
@nuwang That's what surprises me. I would have thought that the .main file was the one to modify, not the sample.
Nuwan Goonasekera
@nuwang
tool_conf.xml.mainrefers to the tool conf on usegalaxy.org which was historically called ‘main’. Admittedly a bit misleading. Perhaps this file is not even needed any more @natefoo?
Nate Coraor
@natefoo:matrix.org
[m]
Yeah, it is not used anymore and should be dropped.
Nuwan Goonasekera
@nuwang
Ok. Will issue a PR
Nate Coraor
@natefoo:matrix.org
[m]
Thanks!
@FiReTiTi: if you have not modified the setting @nuwang referenced (tool_config_file) then you can cp config/tool_conf.xml.sample config/tool_conf.xml and make your modifications there.
Nuwan Goonasekera
@nuwang
Hope the tests don’t use it because there’s still a reference to it there
FiReTiTi
@FiReTiTi
@nuwang @natefoo Thank you for your answers. I didn't see any file config/tool_conf.xml contrary to what was indicated in http://https://galaxyproject.org/admin/tools/add-tool-tutorial/, that's why I tried first to modify the main, and as my test was not found, I tried to modify sample, and decided to ask my question as I found it a little bit confusing.
Glad it ends up being a PR.
natefoo
@natefoo:matrix.org
[m]
Ah yes somewhere we document that you should create tool_conf.xml by copying from the sample if it does not yet exist, but it is missing from that page.
I would recommend using the Galaxy Training Network (training.galaxyproject.org) over that guide, we should probably just remove that page as it's fairly out of date.
Jeevan Karloss
@ajkarloss

I am trying to install galaxy in my MacBook Pro and Getting below errors. Any Tips?

WARNING: The candidate selected for download or install is a yanked version: 'cheetah3' candidate (version 3.2.6.post2 at https://files.pythonhosted.org/packages/7f/ff/d6eebd29ce2d55ce903a268b830302acb05f22f94dff941859776ab79382/Cheetah3-3.2.6.post2-cp36-cp36m-macosx_10_9_x86_64.whl#sha256=62fb67ad64dbe7ec45cf4256745fe4db18245ad335c157c6c788cc3ed24bc6a2 (from https://pypi.org/simple/cheetah3/) (requires-python:>=2.7, !=3.0., !=3.1., !=3.2., !=3.3.))
Reason for being yanked: Failed
Building wheels for collected packages: mercurial
Building wheel for mercurial (setup.py) ... error
ERROR: Command errored out with exit status 1:
command: /Users/jeevka/IRIDA/galaxy/.venv/bin/python -u -c 'import io, os, sys, setuptools, tokenize; sys.argv[0] = '"'"'/private/var/folders/z4/598z4x912_d17v8k299nqtkw0000gn/T/pip-install-k18hs8so/mercurial_da207d2ad9e74fa3b16c10e7785513ba/setup.py'"'"'; file='"'"'/private/var/folders/z4/598z4x912_d17v8k299nqtkw0000gn/T/pip-install-k18hs8so/mercurial_da207d2ad9e74fa3b16c10e7785513ba/setup.py'"'"';f = getattr(tokenize, '"'"'open'"'"', open)(file) if os.path.exists(file) else io.StringIO('"'"'from setuptools import setup; setup()'"'"');code = f.read().replace('"'"'\r\n'"'"', '"'"'\n'"'"');f.close();exec(compile(code, file, '"'"'exec'"'"'))' bdist_wheel -d /private/var/folders/z4/598z4x912_d17v8k299nqtkw0000gn/T/pip-wheel-vrmt_824
cwd: /private/var/folders/z4/598z4x912_d17v8k299nqtkw0000gn/T/pip-install-k18hs8so/mercurial_da207d2ad9e74fa3b16c10e7785513ba/

nsoranzo
@nsoranzo:matrix.org
[m]
@ajkarloss: That error traceback is not very readable, can you paste it on a gist ( https://gist.github.com/ ), please?
Jeevan Karloss
@ajkarloss
Thanks a lot
nsoranzo
@nsoranzo:matrix.org
[m]
@ajkarloss: I think your issue would be solved by galaxyproject/starforge-recipes#81 , which is unfortunately stuck at the moment.
Jeevan Karloss
@ajkarloss
:(
Nate Coraor
@natefoo:matrix.org
[m]
Blech, can we not just remove mercurial at this point? I thought all Galaxy-side usage of mercurial had switched to hgutil
Nate Coraor
@natefoo:matrix.org
[m]
Ah, hg_util still relies on hg on the command line.
FiReTiTi
@FiReTiTi
Hi, I'm a new user, and I'm trying to integrate a pipeline of mine into Galaxy, but I'm having issues with I/Os. The input is a directory, and the outputs are 3 different directories, plus a lot of text going to the stdout and a log file. I read in the documentation how to define the input using <param format="directory".../>and the output with <data format="directory"... />, but is there a tutorial or even examples to help me improve what I have done?
nsoranzo
@nsoranzo:matrix.org
[m]
@ajkarloss The issue I mentioned is solved, you should be able to install now.
Christophe Antoniewski
@drosofff

Hi,
I recently noticed that our galaxy server instance (21.05) are failing to send error reports by email. It used to work before recently (I received some alerts in july).
We are using gmail smtp, so the parameters in our galaxy.yml conf files are these:

galaxy:
    error_email_to: ouradress@gmail.com
    smtp_password: google_app_auth_token
    smtp_server: smtp.gmail.com:587
    smtp_username: ouradress@gmail.com

and the uwsgi log file returns the following error (displayed in the web user interface):

galaxy.util DEBUG 2021-08-29 08:51:59,836 [pN:main.web.1,p:2325,w:1,m:0,tN:uWSGIWorker1Core1] Initiated SSL/TLS connection to SMTP server: smtp.gmail.com:587
galaxy.tools.error_reports DEBUG 2021-08-29 08:52:00,043 [pN:main.web.1,p:2325,w:1,m:0,tN:uWSGIWorker1Core1] Bug report plugin <galaxy.tools.error_reports.plugins.email.EmailPlugin object at 0x7f451875b1d0> generated response ("An error occurred sending the report by email: 'NoneType' object has no attribute 'strip'", 'danger')

I guess the error may come from changes in gmail smtp policy and/or an ssl/tls python module ?

any thought appreciated !

2 replies