I still need to send Frau Thoma-Wittmann possible dates for the SFZ Workshop. Please put fill in the doodle: https://doodle.com/poll/q39hiuz92u77wcki
This could be a nice opportunity to get some more traction and outreach.
I agree with Stephan: please avoid, phenol-chloroform extraction. It smells like hell, is really dangerous and quite sure without a real lab safety instruction not allowed at all with citizens. And even if would be allowed, it needs a bit experience to handle this stuff. Don't do it.
Yeah, that's what I expected kind of. The reason I'm asking is because a friend of mine (an eye doctor) would like to look for a possible "microbiome" inside the eye (anterior chamber behind the pupil), but I'd expect her to recover very low DNA concentrations (if anything useful at all) from this compartment.
Well, the big difference is the amplification step of DNA. When using a nanopore, the DNA is directly sequenced without prior amplification. For Illumina you need to do a PCR before sequencing to amplify the DNA (and introducing biases...)This means: You can start with as little as the DNA-amount from one single cell in case of Illumina. In case of the eye-microbiome I def would do Illumina-based seq...
I've found the description of the amplification-based kits, thank you. They have quite good information.
HI @DonStephano, thanks for the advice. That's pretty much what I thought, but, of course, using nanopore sequencing would add a touch of "they used fancy new technology" to the study so I thought I'll figure out if using it is a real option or not.
Sounds really like a cool project. You may want to stick to Nanopore and make a friend whose patients cry or spit :smile: :smiling_imp:
Hi all, this could be a way to find schools interested in beer sequencing: https://forschungsboerse.de/
New paper probably interesting for us: "An educational guide for nanopore sequencing in the classroom”
Hey, here is the short presentation to introduce us at the galaxy workshop! Feel free to change or propose changes: https://docs.google.com/presentation/d/1nNypWyQI5ZabwNX0v4kPl_t3TcysEFb2BFmBK0HVBH0/edit#slide=id.g5c67b9aa23_0_100
maybe we could apply? 5000 EUR maximun...
Saccharomyces cerevisiae is used as a probiotic in humans and animals. Especially, a strain Saccharomyces cerevisiae var. boulardii is industrially manufactured and clinically used as a medication.[...]
you could also drink just more beer as we know ;)
Exactly, then I thought about the story about boiling beer as a remedy :)
