Thanks for testing the kit! Good to know that it works as it should. Does anyone know how much DNA we used for the library prep?! I mean if there is enough (I know, the more the better) to go on with the library prep, I definitely would prefer the kit based extraction... And a concentration of at least 50 ng/µl doesn't sound bad
Hey, I am not good at these concentration calculations. But for the last sequencing, we used the Rothaus (40 ng/µl). And the sequencing protocol askes for: DNA ~ 400 ng should be in 7.5 µl nuclease-free water. Does this answer your question? Did you get the 50 ng/µl from Martinas numbers?
sorry, my post is a bit mixed up. yes, we had as lowest value 50 ng/µl. The quality is not great according to the Nanodrop and i can't detect the La Trappe DNA on the agarose gel, but that is consistent with the comparatively low amount (when you look at Saccharomyces with >800ng/µl) which by the way looks beautiful.
So i would like to try again improving a bit some incubation steps but the ulitmate test will be to use DNA extracted with the kit for sequencing to really see how well it works. For sure the possibilities for errors is reduced with the kit ;)
Hi. Illumina Foundation and Discovery Education created nice educational resources that could be useful for us: https://www.dnadecoded.org/educator-resources.html
He. There is a webinar from Genewiz on "Brewing with Wild Things: A Genetic Survey of Mixed-Culture Fermentation in the U.S." https://web.genewiz.com/webinar-week-daniel-lepage-3?utm_campaign=Webinar%20Week%202019&utm_source=hs_email&utm_medium=email&utm_content=77912501&_hsenc=p2ANqtz-_2xYMb2tM4baHdBiglfyKYEmMn0qhQVt0YsRsePcf-n_xvPSJNDCQ59TVpgqLOvgv_8TrrGGQQRS5A6dd7ThTwBJgHUZWa8bUC6yi_fAAHVtPMQEXf6HDmAEaK7fmeksVsFoN4&_hsmi=77912774
it will be recorded. so we can still watch it later
Hi @/all. quick reminder. please fill the doodle (https://doodle.com/poll/smw5v2irarw2mbiq) to find a date for our next sequencing day together. thanks :smile:
to know if we can also get a room
we would love to have some feedback on them :smile:
Thank you 😊
Two recent papers studying brewing yeast and their hybrids:
Might be good for inspirational research-oriented ideas
80% pig in seafood product in Singapur https://twitter.com/rudolfmeier15/status/1190651070432890881?s=21
B. bruxellensis has a positive contribution to brewing of Belgium Lambic and Gueuze beers: https://twitter.com/jo_schacherer/status/1191469068181803008?s=21
did the WS take place today?
Having SSD storage and USB3 are some of the most importants.