we had S. cerevisia as positive control - and got nice results. for the yeast from la trappe not so much
Thanks much Martina. How would this compare with the older method? Would you recommend to use the new kit?
Thanks for testing the kit! Good to know that it works as it should. Does anyone know how much DNA we used for the library prep?! I mean if there is enough (I know, the more the better) to go on with the library prep, I definitely would prefer the kit based extraction... And a concentration of at least 50 ng/µl doesn't sound bad
Hey, I am not good at these concentration calculations. But for the last sequencing, we used the Rothaus (40 ng/µl). And the sequencing protocol askes for: DNA ~ 400 ng should be in 7.5 µl nuclease-free water. Does this answer your question? Did you get the 50 ng/µl from Martinas numbers?
sorry, my post is a bit mixed up. yes, we had as lowest value 50 ng/µl. The quality is not great according to the Nanodrop and i can't detect the La Trappe DNA on the agarose gel, but that is consistent with the comparatively low amount (when you look at Saccharomyces with >800ng/µl) which by the way looks beautiful.
So i would like to try again improving a bit some incubation steps but the ulitmate test will be to use DNA extracted with the kit for sequencing to really see how well it works. For sure the possibilities for errors is reduced with the kit ;)
So tomorrow we can also discuss if we do some sequencing soon. :-)